Knock-In of a 25-Kilobase Pair BAC-Derived Donor Molecule by Traditional and CRISPR/Cas9-Stimulated Homologous Recombination
Tiffany Leidy-Davis, Kai Cheng, Leslie O. Goodwin, Judith L. Morgan, Wen Chun Juan, Xavier Roca, Sin-Tiong Ong, David E. Bergstrom
The paper demonstrates the replacement of a 17-kbp mouse Bcl2l11 gene segment with a 25-kbp human BCL2L11 segment using traditional and CRISPR/Cas9 methods, achieving full humanization and functional validation.
bioRxiv · Sep 23, 2016 · PreprintGenetics