Continuous Multiplexed Phage Genome Editing Using Recombitrons
C. B. Fishman, K. D. Crawford, S. Bhattarai-Kline, K. A. Zhang, A. Delgado-Gonzalez, S. L. Shipman
This paper introduces recombitrons, a system for efficient, continuous, and multiplexed phage genome editing without counterselection, achieving high mutation efficiency in lambda phage.
bioRxiv · Mar 24, 2023Bioengineering