Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
P. I. Kulcsar, A. Talas, K. Huszar, Z. Ligeti, E. Toth, N. Weinhardt, E. Fodor, E. Welker
The paper develops HeFSpCas9 variants by combining mutations from eSpCas9 and SpCas9-HF1 to improve specificity and cleavage, showing that no single variant is universally optimal.
bioRxiv · Sep 12, 2017Bioengineering