Role of glutamate 292 and lysine 331 in catalysis for the flavoenzyme (S)-6-hydroxynicotine oxidase from Shinella sp. HZN7
Zhiyao Zhang, Kaitrin Freeland, Frederick W. Stull
This study identifies Glu292 and Lys331 as critical residues in NctB's catalytic mechanism, showing that Glu292 optimizes Lys331's role in dioxygen activation despite lacking the conserved lysine found in other FAO enzymes.
Archives of Biochemistry and Biophysics · May 31, 2025Peer-reviewedBiochemistry