SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and functional studies of D-amino acid oxidase
T. I. K. Munaweera, Nana Odake, Kakeru Ikeda, Bo Zhu, Maurizio Camagna, Tomokazu Ito, Tetsuya Kitaguchi, Naoto Nemoto, Hideo Nakano, Jasmina Damnjanović
The paper introduces SMART, a single-molecule display platform for enzyme engineering, and demonstrates its application in evolving D-amino acid oxidase with improved substrate specificity.
bioRxiv · Mar 23, 2025 · PreprintBioengineering