Do we still need Illumina sequencing data?: Evaluating Oxford Nanopore Technologies R10.4.1 flow cells and v14 library prep kits for Gram negative bacteria whole genome assemblies
N. Lerminiaux, K. Fakharuddin, M. R. Mulvey, L. Mataseje
The paper evaluates Oxford Nanopore Technologies R10.4.1 flow cells and v14 library prep kits for generating high-quality genome assemblies of Gram-negative bacteria, finding that 30X long-read coverage is sufficient with Illumina data, while 100X is needed for long-read-only assemblies.
Canadian Journal of Microbiology · May 1, 2024Peer-reviewedMicrobiology