Efficient RNP-directed human gene targeting reveals SPDEF is required for IL-13-induced mucostasis
Kyung Duk Koh, Sana Siddiqui, Dan Cheng, Luke R. Bonser, Dingyuan Iris Sun, Lorna T. Zlock, Walter E. Finkbeiner, Prescott G. Woodruff, David J. Erle
The paper develops an efficient method for gene targeting in human bronchial epithelial cells using direct delivery of sgRNA and rCas9, demonstrating its application in studying SPDEF's role in IL-13-induced mucostasis.
American Journal of Respiratory Cell and Molecular Biology · Oct 9, 2019Peer-reviewedMolecular Biology