Three-fluorophore FRET enables the analysis of ternary protein association in living plant cells
N. Gloeckner, S. zur Oven-Krockhaus, L. Rohr, F. Wackenhut, M. Burmeister, F. Wanke, E. Holzwart, A. J. Meixner, S. Wolf, K. Harter
The paper uses three-fluorophore FRET and FLIM to show BRI1, BAK1, and RLP44 form a ternary complex in plant plasma membrane nanodomains, distinct from FLS2/BAK1 complexes.
bioRxiv · Oct 26, 2020 · PreprintPlant Biology