12S Gene Metabarcoding with DNA Standard Quantifies Marine Bony FishEnvironmental DNA, Identifies Threshold for Reproducible Amplification, and Overcomes Distortion Due to Non-Fish Vertebrate DNA
M. Y. Stoeckle, J. H. Ausubel, M. Coogan
This study uses an internal DNA standard to quantify marine bony fish eDNA via 12S metabarcoding, showing reproducible amplification above 10 copies per reaction and correcting for non-fish vertebrate DNA distortion.
bioRxiv · Jul 29, 2022 · PreprintEcology