Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge
M. D. Verhoeven, P. H. Nielsen, M. K. D. Dueholm
This paper introduces an experimental method to isolate previously uncultured microbial species from activated sludge using 16S rRNA gene amplicon sequencing, resulting in the cultivation of 102 strains from 200 colonies.
bioRxiv · Jul 13, 2023Microbiology