Zinc-Induced Fluorescence Turn-on in Native and Mutant Phycoerythrobilin-Binding Orange Fluorescent Proteins
G. C. Jensen, M. K. Janis, J. Jara, N. Abbasi, M. L. Zastrow
The paper demonstrates that GAF2-PEB, a fluorescent protein, shows a 5-fold zinc-induced fluorescence enhancement with blue-shifted emission, suggesting a Zn2+ binding site involving bilin cofactor coordination and a nearby cysteine residue.
bioRxiv · Aug 11, 2023Biochemistry