A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation
I. K. Strawn, P. J. Steiner, M. S. Newton, T. A. Whitehead
This paper presents a method to generate user-defined circular and linear single-stranded DNA from plasmids using Golden Gate assembly, nickase, and exonuclease, achieving yields of 33-43% for cssDNA and 52% cleavage efficiency for lssDNA.
bioRxiv · Nov 21, 2022Bioengineering