BiophysicsbioRxiv
Global analysis of protein stability by temperature and chemical denaturation
L. Hamborg, E. W. Horsted, K. E. Johansson…·K. Teilum
This paper introduces a method to assess protein stability using combined temperature and denaturant unfolding with minimal protein requirements, demonstrating its applicability for small-scale protein purification.
DOI 10.1016/j.ab.2020.113863