A bone fragment-based protocol for molecular analysis of osteocyte-associated transcripts in human bone specimens
Chiaki Nishizawa, Soju Seki, Emiko Tanaka Isomura, Mari Namikawa, Kazuma Harada, Yusuke Yokota, Tomonao Aikawa, Susumu Tanaka, Toshimi Michigami, Kazuaki Miyagawa
This paper describes a protocol to isolate osteocyte-associated transcripts from human bone specimens by removing non-osteocytic cells through mechanical and enzymatic steps.
bioRxiv · May 23, 2026 · PreprintCell Biology