In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR
Fee Zimmermann, Maria Urban, Christian Krüger, Mathias C. Walter, Roman Wölfel, Katrin Zwirglmaier
The paper evaluates how mutations in primer binding sites affect RT-qPCR detection of SARS-CoV-2 using synthetic transcripts and identifies mismatches in commercial assays.
Journal of Virological Methods · Nov 5, 2021Peer-reviewedMolecular Biology