Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis
Matteo Carrara, Josephine Lum, Francesca Cordero, Marco Beccuti, Michael Poidinger, Susanna Donatelli, Raffaele A Calogero, Francesca Zolezzi
The paper evaluates how sample prep and bioinformatics analysis affect alternative splicing detection, finding that low-input methods like NuGEN v2 are less effective than high-input methods like TruSeq.
BMC Bioinformatics · Jun 1, 2015Peer-reviewedGenomics