Extraction-free COVID-19 (SARS-CoV-2) diagnosis by RT-PCR to increase capacity for national testing programmes during a pandemic
Paul R. Grant, Melanie Turner, Gee Yen Shin, Eleni Nastouli, Lisa J. Levett
Adding 2 μL of unextracted swab transport medium directly to a SARS-CoV-2 qRT-PCR gave 98.0% sensitivity and 100% specificity against an extraction-based Panther Fusion assay.
bioRxiv · Apr 6, 2020 · PreprintMolecular Biology✦ In-depth digest