Michel Plisnier

Definitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework

This study benchmarks DDA and DIA acquisition modes on the Orbitrap Astral for HCP quantification, finding DIA offers better coverage and sensitivity for low-abundance proteins.

Prospective ICH Q2(R2)-aligned total-error validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics

This paper validates label-free untargeted proteomics for HCP quantification in biotherapeutics using ICH Q2(R2) total-error approach, showing high accuracy and precision across multiple assays and platforms.

Comparative analysis of MS/MS search algorithms in label-free shotgun proteomics for monitoring host-cell proteins using trapped ion mobility and ddaPASEF

This study benchmarks six MS/MS search algorithms for label-free shotgun proteomics of host-cell proteins using trapped ion mobility and ddaPASEF, identifying performance differences in sensitivity, accuracy, and bias.