Genome-wide specificity profiles of CRISPR-Cas Cpf1 nucleases in human cells
Benjamin P Kleinstiver, Shengdar Q Tsai, Michelle S Prew, Nhu T Nguyen, Moira M Welch, Jose M Lopez, Zachary R McCaw, Martin J Aryee, J. Keith Joung
The paper evaluates the genome-wide specificity of two CRISPR-Cas Cpf1 nucleases in human cells, showing high on-target efficiency and low off-target activity for many crRNAs.
bioRxiv · Jun 9, 2016Molecular Biology