Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
J. Tycko, L. A. Barrera, N. Huston, A. E. Friedland, X. Wu, J. S. Gootenberg, O. O. Abudayyeh, V. E. Myer, C. J. Wilson, P. D. Hsu
The paper screens SaCas9 specificity using a barcoded pairwise library of sgRNAs and identifies optimal spacer lengths for off-target activity.
bioRxiv · Feb 22, 2018Bioengineering