Author

J. S. Gootenberg

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Microbiology· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Approach:deep learning· 2 Approach:experimental assay· 2 Organism:human· 2 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1 Research Focus:gene regulatory network inference· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

O. O. Abudayyeh· 2 shared A. E. Friedland· 1 shared A. E. Zepeda· 1 shared A. P. Kadina· 1 shared A. Smargon· 1 shared C. A. Vakulskas· 1 shared C. J. Wilson· 1 shared C. Schmitt-Ulms· 1 shared

Activity by Year

2016-2021 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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2 shared papersBioengineering

O. O. Abudayyeh

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Author profile →

3 papers

Recent·Most cited

Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases

E. I. Ioannidi, M. T. N. Yarnall, C. Schmitt-Ulms, R. N. Krajeski, J. Lim, L. Villiger, W. Zhou, K. Jiang, N. Roberts, L. Zhang, C. A. Vakulskas, J. A. Walker, A. P. Kadina, A. E. Zepeda, K. Holden, , O. O. Abudayyeh

PASTE enables efficient, programmable gene integration without DNA breaks, applicable for research, cell engineering, and gene therapy.

bioRxiv · Nov 1, 2021 · PreprintBioengineering

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

J. Tycko, L. A. Barrera, N. Huston, A. E. Friedland, X. Wu, J. S. Gootenberg, O. O. Abudayyeh, V. E. Myer, C. J. Wilson, P. D. Hsu

The paper screens SaCas9 specificity using a barcoded pairwise library of sgRNAs and identifies optimal spacer lengths for off-target activity.

bioRxiv · Feb 22, 2018Bioengineering

Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNase differentially regulated by accessory proteins Csx27 and Csx28

A. Smargon, D. B. T. Cox, N. Pyzocha, K. Zheng, I. M. Slaymaker, J. S. Gootenberg, O. A. Abudayyeh, P. Essletzbichler, S. Shmakov, K. S. Makarova, E. V. Koonin, F. Zhang

The paper identifies two CRISPR-Cas VI-B systems with Cas13b and characterizes their RNA interference activity and regulation by Csx27 and Csx28.

bioRxiv · Dec 9, 2016Microbiology