The role of ATP hydrolysis in conformational changes of human P-glycoprotein in living cells
R. Futamata, F. Ogasawara, T. Ichikawa, Y. Kimura, N. Kioka, K. Ueda
This study uses FRET to show ATP binding induces the outward-facing conformation of P-gp, while hydrolysis and {gamma}-phosphate release return it to the inward-facing state in living cells.
bioRxiv · Nov 23, 2019Biochemistry