Genome-wide CRISPRi knockdown to map gene essentiality landscape in coliphages {lambda} and P1
D. Piya, N. Nolan, M. L. Moore, L. A. Ramirez Hernandez, B. F. Cress, R. Young, A. P. Arkin, V. K. Mutalik
The paper uses CRISPRi to identify essential genes in coliphages λ and P1, revealing insights into gene essentiality and highlighting potential false positives in less studied phages.
bioRxiv · May 14, 2023 · PreprintMicrobiology