High-speed volumetric single-molecule imaging using dual-wavelength light sheets and PSF-engineered enhanced biplane detection
Prakash Joshi, Nahima Saliba, Siyang Cheng, Yuya Nakatani, Dafei Xiao, Reut Orange Kedem, Yoav Shechtman, Anna‐Karin Gustavsson
This paper introduces SoLiD-3D, a high-speed volumetric SMLM platform that improves localization density and axial resolution using dual-wavelength light sheets and engineered PSFs.
bioRxiv · Jun 25, 2026 · PreprintBiophysics