Metagenomics vs. total RNA sequencing: most accurate data-processing tools, microbial identification accuracy, and implications for freshwater assessments
Christopher A. Hempel, Natalie Wright, Julia Harvie, José Sergio Hleap, Sarah J. Adamowicz, Dirk Steinke
This study compares metagenomics and total RNA-Seq for microbial identification accuracy using 768 workflows, finding total RNA-Seq more accurate at lower sequencing depths and suggesting its potential for cost-effective freshwater assessments.
Nucleic Acids Research · Aug 18, 2022Peer-reviewedGenomics