Dirk Steinke

The paper assesses leaf-litter invertebrate biodiversity using high-throughput sequencing, finding that the Qiagen Blood and Tissue Kit is most effective and that temperature influences community composition.

The paper uses community science and DNA metabarcoding to study cavity-nesting Hymenoptera distributions and trophic interactions, revealing new species associations and range expansions.

The paper analyzes ecological interactions of emerald ash borer beetles using DNA metabarcoding, finding that collection date and life stage best predict interaction detection.

The paper introduces a new primer pair (BirT) for avian eDNA metabarcoding, demonstrating its effectiveness in detecting bird species from water samples with high specificity and taxonomic resolution.

This study shows that DNA metabarcoding of exuviae can identify more insect orders and higher diversity than emergence traps in freshwater habitats.

This paper releases a dataset of 5.6 million arthropod images for training machine learning models in taxonomic identification.

This study compares amplicon sequencing, metagenomics, and total RNA sequencing for predicting environmental stressors using machine learning, finding 16S sequencing outperforms others despite moderate performance.

This study compares metagenomics and total RNA-Seq for reconstructing full-length SSU rRNA from HTS data, finding that total RNA-Seq provides more complete reconstructions.

This study compares metagenomics and total RNA-Seq for microbial identification accuracy using 768 workflows, finding total RNA-Seq more accurate at lower sequencing depths and suggesting its potential for cost-effective freshwater assessments.

This study evaluates seven taxonomic assignment methods for metabarcoding eukaryotes using mock communities, finding that BLAST top hit performs competitively when optimized and that reference database quality impacts accuracy.

This study evaluates the use of Nanopore MinION sequencing for metabarcoding, showing high accuracy with a custom pipeline and RCA amplification.

A2G2 improves alignment accuracy for large sequence sets in conserved regions using MAFFT and a reference-based strategy.

CommDivMap transforms metabarcoding OTU tables into species richness maps, tested on arthropod samples from agricultural plots.