Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells
Keith A. Breau, Meryem T. Ok, Ismael Gomez-Martinez, Joseph Burclaff, Nathan P. Kohn, Scott T. Magness
This paper develops an efficient electroporation method for transgenesis and gene editing in human intestinal stem cells, achieving high efficiency and viability.
Stem Cell Reports · May 5, 2022Peer-reviewedMolecular Biology