In vivo directed evolution of an ultra-fast RuBisCO from a semi-anaerobic environment imparts oxygen resistance
Julie Lynn McDonald, Nathan P. Shapiro, Amanuella A. Mengiste, Sarah Kaines, Spencer Michael Whitney, Robert H. Wilson, Matthew D. Shoulders
This paper uses in vivo directed evolution to improve the oxygen resistance of a fast Rubisco variant from Gallionellaceae, identifying mutations that enhance carboxylation efficiency under ambient oxygen.
Proceedings of the National Academy of Sciences · Jun 30, 2025Peer-reviewedBiochemistry