PiggyBac transposon and transposase delivered in trans, in cis, and as mRNA for engineering CAR-T cells: a multi-objective optimization
Nattanicha Deedit, Koramit Suppipat, Supannikar Tawinwung, Yozo Nakazawa, Nattiya Hirankarn, Thanyavi Chinsuwan
This study compares three piggyBac transposon delivery methods for CAR-T cell engineering, finding that the ONE method offers highest efficiency but lower viability, while MRNA reduces transgene copy numbers at lower efficiency.
bioRxiv · Oct 19, 2026 · PreprintSynthetic Biology