Fluctuations in AAV genome quantification via digital PCR affected by heat-based virion lysis
Antonina Govorov, Filip Trajkovski, Nazguel Wagner, Lucie Klughertz, Marine Guise, Karl B. Pflanz, A. Mueller-Scholz, Robert Hertel
This study shows that shorter amplicons in dPCR lead to higher AAV genome copy numbers, indicating heat-induced fragmentation affects quantification.
bioRxiv · Sep 4, 2025 · PreprintMolecular Biology