Molecular BiologybioRxivPreprint
Fluctuations in AAV genome quantification via digital PCR affected by heat-based virion lysis
Antonina Govorov, Filip Trajkovski, Nazguel Wagner…·Robert Hertel
This study shows that shorter amplicons in dPCR lead to higher AAV genome copy numbers, indicating heat-induced fragmentation affects quantification.
DOI 10.1101/2025.09.04.674220