The mechanism of the nucleo-sugar selection by multi-subunit RNA polymerases
J. J. Mäkinen, Y. Shin, E. Vieras, P. Virta, M. Metsä-Ketelä, K. S. Murakami, G. A. Belogurov
The paper identifies a conserved Arg residue in multi-subunit RNA polymerases that uses a two-pronged strategy to select against 2’dNTPs by interacting with both the 2’OH and 3’OH groups.
bioRxiv · Jun 30, 2020Biochemistry