Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression
Sarah Elizabeth Norred, Rosemary M. Dabbs, Gaurav Chauhan, Patrick M. Caveney, Charles Patrick Collier, Steven M. Abel, Michael L. Simpson
This paper demonstrates that macromolecular crowding and confinement in cell-free systems can spatially organize transcription and translation, mimicking prokaryotic nucleoid behavior.
bioRxiv · Oct 16, 2018 · PreprintSynthetic Biology