Design and Validation of New Primers for Specific and Sensitive Real-time PCR Detection and Quantification of Seven Botulinum Encoding Genes (Serotype A-G) of Clostridium botulinum
Phuong-Lan Phan, Hai-Anh Chu, The-Thai Le, Phan-Anh Le, Hong-Loan Thi Nguyen, Minh Nguyet Tran, Yen Pham, Trung-Thanh Nguyen, Phan Tuấn Nghĩa
This study designs and validates new primers for specific and sensitive real-time PCR detection of seven Botulinum serotypes, achieving 10 copies/reaction sensitivity and eliminating carryover PCR products.
bioRxiv · Sep 1, 2026 · PreprintMolecular Biology