Directed Evolution of Enzymes based on in vitro Programmable Self-Replication
A. Drame-Maigne, R. Espada, G. McCallum, R. Sieskind, Y. Rondelez
This paper develops an in vitro directed evolution method using programmable feedback loops to autonomously select enzyme variants without screening.
bioRxiv · Apr 22, 2021 · PreprintBioengineering