Direct, quantitative, and comprehensive analysis of tRNA acylation using intact tRNA liquid-chromatography mass-spectrometry
R. Fricke, I. Knudson, A. Schepartz
This paper introduces an LC-MS assay to directly and quantitatively analyze tRNA acylation by detecting intact acyl-tRNA products, with sensitivity down to 0.23%.
bioRxiv · Jul 14, 2023Biochemistry