Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia
M. K. Sakata, M. U. Kawata, A. Kurabayashi, T. Kurita, M. Nakamura, T. Shirako, R. kakehashi, K. Nishikawa, M. Y. Hossman, T. Nishijima, J. Kabamoto, M. Miya, T. Minamoto
The paper develops and evaluates PCR primers for eDNA metabarcoding of amphibians, finding that the Amph16S primer set detects twice as many species as physical surveys in agricultural ecosystems.
bioRxiv · Oct 29, 2021 · PreprintEcology