In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE
Misa Shiratori, Rio Tsuyuki, Miwako Asanuma, Saki Kawabata, Hiromasa Yoshioka, Kenji Ohgane
This paper introduces a method to refold fully denatured GFPs within SDS-PAGE gels using cyclodextrin and methanol, enabling in-gel fluorescence detection.
Analytical Biochemistry · Apr 6, 2025Peer-reviewedBiochemistry