Probing the substrate binding-induced conformational change of a ZIP metal transporter using a sandwich ELISA
Yao Zhang, Ryan Hu, Min Su, Jian Qing Hu
This paper develops a sandwich ELISA to study conformational changes in BbZIP upon metal binding, revealing dissociation constants and the role of the M2 site in metal transport.
bioRxiv · Mar 9, 2025 · PreprintBiochemistry