Rigidifying a de novo enzyme increases activity and induces a negative activation heat capacity
S. A. Hindson, A. H. Bunzel, B. Frank, D. A. Svistunenko, C. Williams, M. W. van der Kamp, A. J. Mulholland, C. R. Pudney, R. J. Anderson
This study shows that increasing rigidity in a de novo peroxidase enhances its activity but alters its temperature dependence, resembling natural enzymes.
bioRxiv · Apr 16, 2021Biochemistry