MePMe-seq: Antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing
K. Hartstock, A. Ovcharenko, N. A. Kueck, P. Spacek, N. V. Cornelissen, S. Huewel, C. Dieterich, A. Rentmeister
MePMe-seq detects m6A and m5C mRNA modifications simultaneously without antibodies or sequence motifs, enabling single-nucleotide precision in the same sequencing run.
bioRxiv · Mar 16, 2022Biochemistry