A practical guide to time-resolved fluorescence microscopy and spectroscopy
B. S. Clark, I. Silvernail, K. Gordon, J. F. Castaneda, A. N. Morgan, L. A. Rolband, S. J. LeBlanc
This paper provides a guide to calibrating FCS and PIE-FRET measurements using biological samples to determine diffusion coefficients and FRET efficiency.
bioRxiv · Jan 25, 2024 · PreprintBiophysics