Exonuclease III (XthA) enforces in vivo DNA cloning of Escherichia coli to create cohesive ends
This paper identifies XthA as the key exonuclease enabling in vivo DNA cloning in E. coli, demonstrating a mechanism involving 3' end resection and gap filling by DNA polymerase I.
bioRxiv · Oct 26, 2018Bioengineering