NPlasmid-seq: Cas9-encoded terminal signatures enable molecule-level nanopore quality control of vector and IVT-template plasmids
Yan Xu, Wei Wen, Di Sun, Yi-Xue Yin, Xiao-Yu Ma, Jia-Xin Ren, Shu-Man Zhang, Jian-Qiao Chang, Shan-Chen Pang, Shu-Dong Wang, Xiao-Bing Zhang, Jian-Ping Zhang
NPlasmid-seq detects sub-percent contamination and sequence heterogeneity in plasmid preparations using Cas9-encoded terminal signatures, revealing structural variants missed by consensus-based methods.
bioRxiv · Nov 3, 2026 · PreprintBioengineering