Optimizing multiplex CRISPR/Cas9-based genome editing for wheat
Wei Wang, Alina Akhunova, Shiaoman Chao, Eduard Akhunov
The paper develops a multiplex CRISPR/Cas9 strategy for wheat genome editing using tRNA-gRNA units and validates its efficiency through protoplast assays and NGS.
bioRxiv · May 12, 2016 · PreprintGenomics