Author

Shirou Itagaki

Sapporo Medical University

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Molecular Biology· 2

Signature Assays, Organisms & Methods

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Organism:E. coli· 2 Approach:experimental assay· 2 Organism:mouse· 1 Research Focus:protein design· 1 Research Focus:protein structure prediction· 1 Assay:proteomics· 1

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Agnieszka M. Murakami· 2 shared Kazuyoshi Hirota· 2 shared Manabu MurakamiiD· 2 shared

Activity by Year

2020-2021 · peak 1/yr

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2 shared papersMolecular Biology

Agnieszka M. Murakami

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2 papers

Recent·Most cited

A dual prokaryotic (E. coli) expression system (pdMAX)

Manabu Murakami, Agnieszka M. Murakami, Kazuyoshi Hirota, Shirou Itagaki

The paper introduces a dual promoter plasmid system (pdMAX) for expressing two tagged proteins in E. coli, with subcloning and expression capabilities.

PLoS ONE · Oct 21, 2021Peer-reviewedMolecular Biology

A new dual prokaryotic (E. coli) and mammalian expression system (pgMAXs)

Manabu Murakami, Agnieszka M. Murakami, Kazuyoshi Hirota, Shirou Itagaki

The paper introduces a new plasmid system (pgMAXs) for efficient subcloning and expression in both E. coli and mammalian cells, with features for protein tagging and epitope identification.

bioRxiv · May 21, 2020 · PreprintMolecular Biology