To join or not to join: handling biological replicates in long-read RNA sequencing data
Fabian Jetzinger, Alejandro Paniagua, Stanley Cormack, Jorge Mestre‐Tomás, José Manuel Morante‐Redolat, Isabel Fariñas, Luis Ferrández-Peral, Stefan Götz, Carolina Monzó, Ana Conesa
This study compares two strategies for combining biologically replicated long-read RNA-seq data to identify transcripts, finding that 'Join & Call' is better for novel isoforms while 'Call & Join' is more efficient for large datasets.
bioRxiv · Dec 12, 2025 · PreprintBioinformatics