Coupling multiplex pre-amplification and droplet digital PCR for longitudinal monitoring of ESR1 and PIK3CA mutations from plasma cell-free DNA
Huilan Yao, Grant Wu, Subhasree Das, Crystal J. MacKenzie, Hua Gao, Victoria M. Rimkunas, Zhaojie Zhang, Stephanie L Ferro, Amy Roden, Manav Korpal, Joanne Schindler, Peter G. Smith, Lihua Yu, Ping Zhu, Pavan Kumar
This paper develops a method combining multiplex pre-amplification and ddPCR to detect ESR1 and PIK3CA mutations in plasma cfDNA from MBC patients, achieving high sensitivity and reproducibility.
bioRxiv · Apr 5, 2019 · PreprintGenetics