Complementary Single-Cell Microflow HILIC and Ion Pair LC-MS Reveal Bystander Metabolic Effects in a Macrophage Model of Tuberculosis
Abigail Cook, Rahul Deshpande, Abigail E. Ellis, Ryan D. Sheldon, Claire Davison, Jordan Pascoe, Susan Bird, Dany J. V. Beste, Melanie Jane Bailey
The paper develops and applies complementary microflow HILIC and ion pair LC-MS methods to analyze single-cell metabolomics in a tuberculosis macrophage model, revealing infection-associated metabolic changes and a distinct bystander phenotype.
bioRxiv · Jun 23, 2026 · PreprintMicrobiology