EcologybioRxiv
Dataset complexity impacts both MOTU delimitation and biodiversity estimates in eukaryotic 18S rRNA metabarcoding studies
A. De Santiago, T. J. Pereira, S. L. Mincks, H. M. Bik
This study evaluates how bioinformatics tools affect biodiversity estimates and MOTU delimitation in eukaryotic 18S rRNA metabarcoding, finding that ASV algorithms like DADA2 provide more accurate results than OTU clustering methods.
DOI 10.1002/edn3.255